Journal of Clinical and Translational Hepatology

Journal of Clinical and Translational Hepatology

Friday, 09 / 21 / 2018

Articles

Searching for Bacterial Biofilm in Recurrent Cholangitis in Primary Sclerosing Cholangitis: A Case Presentation and Introduction of an Unexplored Disease Mechanism

CASE REPORT

Searching for Bacterial Biofilm in Recurrent Cholangitis in Primary Sclerosing Cholangitis: A Case Presentation and Introduction of an Unexplored Disease Mechanism

Alyssa Y. Choi1, Florencia Jalikis2, Maria Westerhoff2, Sarag Boukhar3, Elinor Pulcini4, Chris Damman5 and Lei Yu*,5

Journal of Clinical and Translational Hepatology 2018;6(1):114-118 DOI: 10.14218/JCTH.2017.00029
Received: April 29, 2017 Accepted: November 11, 2017 Published online: December 17, 2017

1Department of Internal Medicine, University of Washington, Seattle, Washington, USA
2Department of Pathology, University of Washington, Seattle, Washington, USA
3Department of Pathology, University of Iowa, Iowa City, Iowa, USA
4Center for Biofilm Engineering, Montana State University, Bozeman, Montana, USA
5Division of Gastroenterology, University of Washington, Seattle, Washington, USA

*Correspondence to: Lei Yu, Division of Gastroenterology, University of Washington, 1959 NE Pacific Street, Box 356175, Seattle, WA 98195-6175, USA. Tel: +1-206-598-2212, Fax: +1-206-598-3884, E-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

Abstract

Inflammation and fibrosis of the bile ducts are the defining pathological characteristics of primary sclerosing cholangitis (PSC). A previously unexplored mechanism for recurrent cholangitis, one of PSC’s most common presentations, is bacterial colonization of the biliary epithelium in the form of biofilm, which may confer resistance to antibiotics and host phagocytic machinery. The aim of the current study was to assess whether bacteria could be seen on the liver explant and whether they organized in the form of biofilm. An explanted PSC liver from a 60-year-old male who suffered from recurrent cholangitis was formalin-fixed, paraffin-embedded and Gram stained. The specimens were observed under light microscopy. Neither bacteria nor biofilm were detected. We did not detect bacteria or biofilm in the liver explant of a single PSC patient with recurrent cholangitis using standard light microscopy. We suspect this may be in part due to techniques related to tissue preservation and microscopy.

Keywords

Primary sclerosing cholangitis, Recurrent cholangitis, Biofilm

 

 

Journal of Clinical and Translational Hepatology 2018 vol. 6, 114-118  [ Html ] [ PDF Full-text ]

© The Authors 2018. This article is published under the terms of the Creative Commons Attribution-Noncommercial License (CC BY-NC 4.0), which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license.

 

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